F3133

Fibroblast Growth Factor from bovine pituitary

lyophilized, suitable for cell culture

• 别名: FGF
• CAS号: 62031-54-3
• MDL號碼: MFCD00166724
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• product name: Fibroblast Growth Factor from bovine pituitary, suitable for cell culture
• 生物源: bovine pituitary glands
• 品質等級: 200
• 形狀: lyophilized powder
• 效力: 1-100 ng/mL
• 分子量: 16.4 kDa
• 包裝: pkg of 10 and 5X10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: endotoxin, tested
• 顏色: white to off-white
• 儲存溫度: −20°C

说明

一般說明

Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.

應用

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.

Fibroblast Growth Factor from bovine pituitary has been used in in vitro testicular cell culture. It has also been used as a component of myogenic culture medium.

生化／生理作用

Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis and organogenesis. It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells. Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival. FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.

品質

Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.

外觀

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.

準備報告

Purified glands by a modification of the method of Gospodarowicz.

分析報告

The biological activity is measured using a 3-day MTT assay.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)