推荐产品
生物源
mouse
共軛
FITC conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
UCHT-4, monoclonal
形狀
buffered aqueous solution
物種活性
human
技術
flow cytometry: 10 μL using 1 × 106 cells
同型
IgG2a
UniProt登錄號
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
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一般說明
Monoclonal Anti-Human CD8 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with human thymocytes followed by peripheral blood T cells. The CD8 antigen is strongly expressed on approximately one-third of mature T cells (cytotoxic/suppressor T cells). Monoclonal Anti-CD8 does not stain B lymphocytes, monocytes, or granulocytes.
特異性
Recognizes the CD8 human T cytotoxic/suppressor lymphocyte cell surface glycoprotein. Approximately 90% of thymocytes are stained in suspension. In thymus sections, both cortical and medullary thymocytes are stained. A subset of NK cells express this antigen weakly. The epitope recognized by this clone is sensitive to routine formalin fixation and paraffin embedding.
3rd Workshop: code no. 567
3rd Workshop: code no. 567
免疫原
human thymocytes followed by peripheral blood T cells.
應用
FITC Monoclonal Anti-Human CD8 may be used for:
- Immunofluorescent staining,
- Enumeration of total T cytotoxic/suppressor lymphocytes in bone marrow, blood and other body fluids,
- Identification and localization of T cytotoxic/ suppressor lymphocytes in lymphoid and other tissues,
- Analysis of cell mediated cytotoxicity,
- Studies of immunoregulation in health and disease,
- Investigation of NK cells, Complement mediated cytolysis of CD8 expressing cells
生化／生理作用
CD8a (Cluster of Differentiation 8a) is a human gene. CD8 bind to class I MHC and the co-receptors during the process of cytotoxic T-cell activation. T cells that express T cell receptor (TCR) complexes, which lack CD3 δ, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. CD3 δ couples with TCR. The CD3 associated with CD8 is required for effective activation and positive selection of CD8+ T cells. CD8 expression is a recurrent rare phenomenon in patients with chronic lymphocytic leukemia (CLL).
標靶描述
CD8 is a two chain complex (α/α, α/β) receptor for Class I MHC molecules. The receptor′s cytoplasmic portion is associated with p56lck protein tyrosine kinase which phosphorylates nearby proteins. The antigen is strongly expressed on approximately one third of mature T cells (cytotoxic/suppressor T cells).
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
準備報告
Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Stefan Mehrle et al.
Immunology and cell biology, 83(1), 33-39 (2005-01-22)
Signalling lymphocyte activation molecule (SLAM)-associated protein (SAP) is a small protein that is mutant in humans with X-linked lymphoproliferative (XLP) disease. Patients with XLP disease are affected by fatal EBV infection and malignant B-cell lymphomas. The increased risk for B-cell
Stefan Mehrle et al.
Molecular immunology, 45(3), 796-804 (2007-08-19)
Signaling lymphocyte activation molecule (SLAM, CD150) is a co-stimulatory receptor involved in T cell activation. The activity of CD150 is dependent on the intracellular signaling molecule SAP. Here, we investigated anti-CD3 activated human lymphocytes, transfected either with CD150-plasmid or with
G F Gao et al.
Immunology today, 21(12), 630-636 (2000-12-15)
In recent years, substantial progress has been made towards understanding the molecular basis for CD8 binding to class I MHC and the coreceptor's role in cytotoxic T-cell activation. Here, we review the structural, mechanistic and functional studies that point to
Wolfgang Kern et al.
Cytometry. Part B, Clinical cytometry, 82(3), 145-150 (2011-12-07)
In patients with chronic lymphocytic leukemia (CLL), aberrant expression of the T-lineage antigen CD8 was reported in low frequencies. The clinical impact of this phenomenon remains in discussion. We analyzed 5,523 patients with CLL (21-97 years) by multiparametric flow cytometry
Marie-Agnès Doucey et al.
The Journal of biological chemistry, 278(5), 3257-3264 (2002-09-07)
T cells expressing T cell receptor (TCR) complexes that lack CD3 delta, either due to deletion of the CD3 delta gene, or by replacement of the connecting peptide of the TCR alpha chain, exhibit severely impaired positive selection and TCR-mediated
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