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應用
牛脾脱氧核糖核酸酶Ⅱ已用于:
- 作为从结肠样品分离免疫细胞的消化混合液组分
- 消化视网膜外植体以制备单细胞悬液
- 分离人结肠组织的基质细胞
- 作为从大鼠心脏制备胚胎心肌细胞的解离培养基组分
生化/生理作用
脱氧核糖核酸酶II,也称为酸性DNA酶,可水解天然和变性DNA中的脱氧核糖核苷酸连接键,产生带有3′-磷酸的产物。体外最佳pH范围是4.5-5.0。它还可在pH 5.6-5.9下作用于对硝基苯基磷酸二酯。分子量约为38,000 Da。
單位定義
在 pH 4.6 25℃条件下,一个Kunitz单位可每分钟在每毫升内产生0.001的ΔA260;[Mg2+] = 0.83 mM
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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