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Merck

D3385

Sigma-Aldrich

3'-去磷酸辅酶 A

≥90% (HPLC)

别名:

3′-脱磷酸辅酶 A, dpCoA

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About This Item

经验公式(希尔记法):
C21H35N7O13P2S
CAS号:
分子量:
687.55
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.51

生物源

synthetic (organic)

品質等級

化驗

≥90% (HPLC)

形狀

powder

雜質

≤1% CoA

溶解度

H2O: soluble-50 mg/mL, clear to slightly hazy, colorless to faintly yellow

儲存溫度

−20°C

SMILES 字串

CC(C)(COP(O)(=O)OP(O)(=O)OCC1OC(C(O)C1O)n2cnc3c(N)ncnc23)C(O)C(=O)NCCC(=O)NCCS

InChI

1S/C21H35N7O13P2S/c1-21(2,16(32)19(33)24-4-3-12(29)23-5-6-44)8-39-43(36,37)41-42(34,35)38-7-11-14(30)15(31)20(40-11)28-10-27-13-17(22)25-9-26-18(13)28/h9-11,14-16,20,30-32,44H,3-8H2,1-2H3,(H,23,29)(H,24,33)(H,34,35)(H,36,37)(H2,22,25,26)

InChI 密鑰

KDTSHFARGAKYJN-UHFFFAOYSA-N

應用

3′-脱磷酸辅酶 A 已被用作启动转录的底物。
脱磷酸辅酶 A 是辅酶 A 的直接前体。通过双功能 CoA 合成酶(CoASy,4′-磷酸泛素腺苷基转移酶/去磷酸-CoA 激酶),其负责 γ-干扰素的转移-ATP 的磷酸盐转化为去磷酸辅酶 A。

生化/生理作用

3′-脱磷酸辅酶 A (DepCoA) , 也称为脱磷酸辅酶 A (dpCoA) , 是由 4-磷酸泛酰乙胺通过磷酸泛酰乙胺腺苷基转移酶合成。是辅酶 A 生物合成途径中的一种中间体。在去磷酸 CoA 激酶 (CoaE) 存在下,去磷酸辅酶 A 转化为辅酶 A。3′-脱磷酸辅酶与嘌呤类一样共享腺苷二磷酸亚结构,是一种非经典核苷酸,是转录起始的启动因子。 体外 转录研究中常规用于启动 RNA 合成。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Guneet Walia et al.
PloS one, 6(1), e15228-e15228 (2011-01-26)
Dephosphocoenzyme A kinase performs the transfer of the γ-phosphate of ATP to dephosphocoenzyme A, catalyzing the last step of coenzyme A biosynthesis. This enzyme belongs to the P-loop-containing NTP hydrolase superfamily, all members of which posses a three domain topology
CapZyme-Seq Comprehensively Defines Promoter-Sequence Determinants for RNA 5? Capping with NAD+
Vvedenskaya IO, et al.
Molecular Cell, 70(3), 553-564 (2018)
L Luo et al.
Biochemistry, 40(51), 15684-15692 (2001-12-19)
4-Chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of 4-CBA-CoA to 4-hydroxybenzoyl-CoA (4-HBA-CoA) via a multistep mechanism involving initial attack of Asp145 on C(4) of the substrate benzoyl ring to form a Meisenheimer intermediate (EMc), followed by expulsion of the
Efficient incorporation of CoA, NAD and FAD into RNA by in vitro transcription
Huang F
Nucleic Acids Research, 31(3), e8-e8 (2003)
A Geerlof et al.
The Journal of biological chemistry, 274(38), 27105-27111 (1999-09-10)
Phosphopantetheine adenylyltransferase (PPAT) catalyzes the penultimate step in coenzyme A (CoA) biosynthesis: the reversible adenylation of 4'-phosphopantetheine yielding 3'-dephospho-CoA and pyrophosphate. Wild-type PPAT from Escherichia coli was purified to homogeneity. N-terminal sequence analysis revealed that the enzyme is encoded by

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