D219

Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

• 别名: Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3
• MDL號碼: MFCD01323189
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: ascites fluid
• 抗體產品種類: primary antibodies
• 無性繁殖: 20A, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 143 kDa (reduced); antigen 220 kDa (non-reduced)
• 物種活性: rat, mouse, rabbit, guinea pig
• 技術: immunohistochemistry (frozen sections): 1:500; western blot: 1:500
• 同型: IgG2a
• UniProt登錄號: O08532
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Cacna2d1(12293) ; rat ... Cacna2d1(25399)

说明

一般說明

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

特異性

Reacts specifically with the α₂ subunit of the DHP receptor. Strong reactivity is observed with the α₂ subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

免疫原

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:

• immunofluorescent histochemistry
• surface plasmon resonance binding
• immunohistochemistry (IHC)
• immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

生化／生理作用

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

外觀

Solution in phosphate buffered saline containing 0.05% sodium azide.

其他說明

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)