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Merck
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主要文件

CMC0009

Sigma-Aldrich

STEADY电感受态细胞

Escherichia coli, rod shaped

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About This Item

分類程式碼代碼:
12352200
生物源:
Escherichia coli
增長模式:
adherent or suspension
形態學:
rod shaped

产品名称

STEADY电感受态细胞, for plasmid production using unstable DNA

生物源

Escherichia coli

等級

for molecular biology

形狀

buffered aqueous solution

增長模式

adherent or suspension

形態學

rod shaped

技術

microbiological culture: suitable

細胞轉化

competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

特點和優勢

  • Clone unstable sequences with reliable results
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 × 108 cfu/μg (chem) or 1 × 1010 cfu/μg (electro)
  • Excellent value

成分

  • STEADY electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

儲存類別代碼

10 - Combustible liquids


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分析证书(COA)

Lot/Batch Number

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