CMC0008

STEADY 化学感受态细胞 

Escherichia coli, rod shaped

• 分類程式碼代碼: 12352200

属性

• product name: STEADY 化学感受态细胞 , for plasmid production using unstable DNA
• 生物源: Escherichia coli
• 等級: for molecular biology
• 形狀: buffered aqueous solution
• 增長模式: adherent or suspension
• 形態學: rod shaped
• 技術: microbiological culture: suitable
• 細胞轉化: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• 運輸包裝: dry ice
• 儲存溫度: −70°C

说明

一般說明

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

特點和優勢

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

成分

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

安全信息

• 儲存類別代碼: 10 - Combustible liquids