Eos-HL-60

96100920, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: Eos-HL-60, 96100920
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian promyelocytic leukaemia

細胞系描述

Eos-HL-60 is a variant of the promyelocytic cell line HL60 established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling

應用

Study of production and function of human eosinophils, analysis of differentiation.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

培養基

IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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