TT

92050721, human thyroid, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: TT, 92050721
• 生物源: human thyroid
• 增長模式: Adherent
• 染色體組型: Aneuploid karyotype with marker chromosomes
• 形態學: Not specified
• 產品: Calcitonin, Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian thyroid medullary carcinoma

細胞系描述

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

培養基

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

其他說明

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