TGP 54

94022430, mouse pancreas, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: TGP 54, 94022430
• 生物源: mouse pancreas
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Somatostatin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse pancreatic islet cell tumour

細胞系描述

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

應用

Study of exocrine pancreas carcinomas, hormone secretion studies.

培養基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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