PORTHOS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: Porcine thyroid
• 包裝: tube of 5 μg 93122323-DNA-5UG; pkg of vial of cells 93122323-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Thyroglobulin, plasminogen activator (u-PA)
• 受體: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Porcine thyroid epithelial

細胞系描述

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

應用

Regulation of thyroid cell metabolism

培養基

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 ⁴ cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

其他說明

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