SCL 4.1/F7

93031204, rat nerve (sheath), Flattened or crescent shaped

• 分類程式碼代碼: 41106514

属性

• product name: SCL 4.1/F7, 93031204
• 生物源: rat nerve (sheath)
• 增長模式: Adherent
• 染色體組型: 42,XY / 43,XY+7
• 形態學: Flattened or crescent shaped
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Schwann cell

細胞系描述

This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.

培養基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C Saturation density is 10,000 cells/cm².

其他說明

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