ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

• 分類程式碼代碼: 41106514

属性

• product name: ND8/34 Cell Line from mouse, 92090904
• 生物源: Mouse x Rat Hybridoma nerve
• 描述: Mouse neuroblastoma x Rat neurone hybrid
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse neuroblastoma x Rat neurone hybrid

細胞系描述

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

應用

Study of neuronal differentiation.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

其他說明

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