BCL1 Clone CW13.20-3B3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse blood
• 包裝: tube of 5 μg 90061904-DNA-5UG; pkg of vial of cells 90061904-1VL
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c B cell leukaemia

細胞系描述

Derived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.

應用

B-cell differentiation studies

培養基

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates acc

其他說明

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