C127I

90060504, mouse breast, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: C127I, 90060504
• 生物源: mouse breast
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse RIII mammary tumour

細胞系描述

Non-transformed clone from a RIII mouse. Supernatant fluid is negative for RT activity. Useful for visualisation of sarcoma virus-induced foci, in vitro quantitative assay for bovine papilloma virus. Suitable host for transformation with bovine papilloma virus DNA plasmids.

應用

Virus and transformation studies: Sarcoma virus, Bovine papilloma virus

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:15 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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