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product name
LL29 (AnHa), 87112508
生物源
human lung
增長模式
Adherent
染色體組型
2n = 46; diploid
形態學
Fibroblast-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human Caucasian diploid lung
細胞系描述
Derived from a biopsy taken from a 26 year old Caucasian male with idiopathic pulmonary fibrosis.
應用
Tumourigenicity studies
培養基
Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. A further 9 population doublings expected.
其他說明
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