KG-1

86111306, human bone marrow, Myeloblast-like

• 别名: KG1 Cells
• 分類程式碼代碼: 41106514

属性

• 产品名称: KG-1, 86111306
• 生物源: human bone marrow
• 增長模式: Suspension
• 染色體組型: 2n = 46, diploid
• 形態學: Myeloblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian bone marrow myelogenous leukaemia

細胞系描述

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

應用

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

培養基

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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