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生物源
Rat x Rat Hybridoma
ImmunoDon
AO rat spleen
骨髓瘤
Y3Ag1.2.3
反應
Anti-Glycoprotein of Chlamydomonas reinhardii
技術
cell culture | mammalian: suitable
同型
IgM
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat Y3Ag1.2.3 x Rat AO spleen
細胞系描述
The antibody recognises the glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 14 shows similar fine specificity and properties to AFRC MAC 12, 13, 15, 16 and 17.
免疫原
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
培養基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
其他說明
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