IMR-90 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020204, human lung (fetal), Fibroblast

• 别名: I90 Cells, IMR90 Cells, Institute for Medical Research-90 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human lung (fetal)
• 描述: Human Caucasian fetal lung fibroblast
• 包裝: tube of 5 μg 85020204-DNA-5UG; pkg of vial of cells 85020204-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 46, diploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian foetal lung fibroblast

細胞系描述

Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.

應用

IMR-90 human cell line has been used to study the cytotoxicity of hydroxyapatite/gold/arginine (HAp/Au/arginine) nanoparticles.

Virus studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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