BHK21C13-3P

84100501, hamster kidney, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: BHK21C13-3P, 84100501
• 生物源: hamster kidney
• 增長模式: Adherent
• 染色體組型: 2n = 44, pseudodiploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Hamster Syrian kidney

細胞系描述

BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.

應用

Virus replication studies

培養基

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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