A9(Hamprecht)

8062526, mouse connective tissue, Fibroblast

• 分類程式碼代碼: 41106514

属性

• 产品名称: A9(Hamprecht), 08062526
• 生物源: mouse connective tissue
• 增長模式: Adherent
• 染色體組型: Modal No of chromosomes is 54
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse Fibroblast (L cells)

細胞系描述

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

應用

Used in the generation of cell hybrids.

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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