KARPAS 299

6072604, human lymph, Lymphoblastoid

• 别名: K299 Cells, KARPAS299 Cells
• 分類程式碼代碼: 41106514

属性

• product name: KARPAS 299, 06072604
• 生物源: human lymph
• 增長模式: Suspension
• 染色體組型: Hypodiploid
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Non-Hodgkin′s Ki-positive Large Cell Lymphoma

細胞系描述

A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin′s lymphoma in 1986.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 7,8.3
TPOX: 8
vWA: 17,19

培養基

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

例行更新培養

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.