MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• 别名: MDCK-SIAT1 Cells
• 分類程式碼代碼: 41106514

属性

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• 生物源: canine kidney
• 描述: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Enhanced expression of 6-linked sialic acids
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

細胞系描述

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

培養基

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

其他說明

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