RCC4plusVHL

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 3112703, human kidney, Epithelial

• 别名: RCC4 VHL(+) Cells, RCC4-pVHL Cells, RCC4/VHL Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human kidney
• 包裝: tube of 5 μg 03112703-DNA-5UG; pkg of vial of cells 03112703-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL

細胞系描述

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line (Sigma catalogue no. 03112702) can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.

應用

Study of tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. Cell responses to hypoxia.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

培養基

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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