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Merck

C6788

Sigma-Aldrich

4-氯-1-萘酚

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

别名:

4-CN, 4-chloronaphthalen-1-ol

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About This Item

线性分子式:
ClC10H6OH
CAS号:
分子量:
178.61
Beilstein:
509750
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

4-氯-1-萘酚, tablet

形狀

tablet

品質等級

mp

118-121 °C (lit.)

溶解度

methanol: 10 mL, clear, colorless

儲存溫度

−20°C

SMILES 字串

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI 密鑰

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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相关类别

特異性

4-氯-1-萘酚是一种适合用于免疫印迹程序的过氧化物酶底物。该底物产生的不溶性最终产物为蓝色,可目视观察。

應用

4-氯-1-萘酚已被用作酶联免疫斑点(ELISpot)试验的底物,用于检测斑点。它还被用作沉淀生色底物,用于免疫印迹后抗原−抗体反应性条带的成像。

數量

每片含 30 mg 基质。

重構

将 1 片片剂溶于 10 mL 甲醇中。向 10 mL 三乙醇胺缓冲盐水 (pH 7.5) 中加入 2 mL 甲醇贮备液。临用前加入 5μl 新鲜 30% 过氧化氢(Sigma 产品编号:H-1009)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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Precolostral serology in calves born from Neospora-seropositive mothers
Staubli D, et al.
Parasitology Research, 99(4), 398-404 (2006)
Daniela Staubli et al.
Parasitology research, 99(6), 648-658 (2006-05-24)
Neospora caninum ranges among the major causes of infectious abortion in cattle worldwide. The present study was designed to improve the serodiagnostic tools by complementing a conventional ELISA with a highly sensitive and species-specific N. caninum immunoblot. To evaluate this
Karl Albert Brokstad et al.
The Journal of infectious diseases, 185(7), 878-884 (2002-03-29)
The immune response in the nasal mucosa to influenza vaccination in 23 patients scheduled for tonsillectomy was studied. A statistically significant increase in influenza virus-specific serum and oral fluid antibodies was observed 7 days after vaccination. The numbers of influenza
H Ellakany et al.
Avian pathology : journal of the W.V.P.A, 27(6), 547-554 (2008-05-17)
Two experiments were conducted to test the sensitivity of Western blotting for detection of M. gallisepticum antibodies in respiratory washings and sera of infected chickens by mouse monoclonal antibodies to chicken IgG, IgM and IgA. In the first experiment, birds
C F Frey et al.
Parasitology research, 104(6), 1269-1277 (2009-01-09)
Trichinellosis is a zoonotic disease in humans caused by Trichinella spp. According to international regulations and guidelines, serological surveillance can be used to demonstrate the absence of Trichinella spp. in a defined domestic pig population. Most enzyme-linked immunosorbent assay (ELISA)

商品

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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