C6357

Caspase 4 human

recombinant, expressed in E. coli, 5,000 units/mg protein

• 别名: ICE(rel)II, Ich-2, TX
• EC 号: 3.4.22.57 (BRENDA, IUBMB)
• MDL號碼: MFCD03456356
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 重組細胞: expressed in E. coli
• 形狀: solution
• 比活性: 5,000 units/mg protein
• 分子量: 30 kDa
• 溶解度: PBS: soluble
• UniProt登錄號: P49662
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CASP4(837)

说明

一般說明

The caspase 4 (CASP4) gene is mapped to human chromosome 11q21. Caspase 4 is a cysteine-aspartic protease, localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).

生化／生理作用

Caspase 4 is an inflammatory caspase associated with innate immune responses. It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).

Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

單位定義

One unit will hydrolyze 1 nmol of the caspase substrate WEHD-pNA to WEHD and p-nitroaniline per hour at pH 7.2 at 37 °C.

外觀

Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable