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Key Documents

C5405

Sigma-Aldrich

鸡血清

USA origin, sterile-filtered, suitable for cell culture

别名:

鸡血清

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

生物源

chicken

無菌

sterile-filtered

形狀

liquid

成份

hemoglobin, ≤60 mg/dL

來源

USA origin

技術

cell culture | mammalian: suitable

雜質

≤50 EU/mL endotoxin

運輸包裝

dry ice

儲存溫度

−20°C

應用

鸡血清在寄生虫学中用于研究 microphalid 吸虫 Maritrema novaezealandensis 的排囊、生长、存活和产卵。 鸡血清用于鸡角质形成细胞的连续培养。
鸡血清已用于:
  • 离心沉淀重悬,用于制备酵母聚糖悬液进行呼吸爆发试验
  • 作为Dulbecco′s改良Eagle′s培养基(DMEM)补充剂,培养HeLa P细胞
  • 免疫荧光(IF)双染色法的封闭

生化/生理作用

动物血清可作为基础细胞培养基补充剂的经济替代品。常用于在免疫组化(IHC)、免疫细胞化学(ICC)和蛋白质免疫印迹等免疫检测实验中封闭非特异性结合。鸡血清在寄生虫学中用于研究吸虫Maritrema novaezealandensis(microphallid)的排囊、生长、存活和产卵。可用于鸡角质形成细胞的连续培养。

分析報告

内毒素检测

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Géraldine Rouquet et al.
Journal of bacteriology, 191(13), 4427-4440 (2009-04-21)
We identified a carbohydrate metabolic operon (frz) that is highly associated with extraintestinal pathogenic Escherichia coli (ExPEC) strains. The frz operon codes for three subunits of a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) transporter of the fructose subfamily, for a transcriptional activator
In vitro cultivation of Maritrema novaezealandensis (Microphallidae): the effect of culture medium on excystation, survival and egg production.
Fredensborg BL, Poulin R.
Parasitology Research, 95, 10-13 (2005)
Tom W Anderson et al.
Molecular biology of the cell, 19(11), 5006-5018 (2008-09-19)
In migrating fibroblasts actomyosin II bundles are graded polarity (GP) bundles, a distinct organization to stress fibers. GP bundles are important for powering cell migration, yet have an unknown mechanism of formation. Electron microscopy and the fate of photobleached marks
Amandine Vanhoutteghem et al.
Differentiation; research in biological diversity, 72(4), 123-137 (2004-05-26)
The epidermis of birds differs from that of mammals by the presence of intracellular lipid droplets and the absence of sebaceous glands. We describe here the cultivation of chicken epidermal keratinocytes; these cells cannot be grown in medium supplemented with
Marc A J Morgan et al.
PloS one, 8(2), e56738-e56738 (2013-02-19)
The spontaneous destruction of insulin producing pancreatic beta cells in non-obese diabetic (NOD) mice provides a valuable model of type 1 diabetes. As in humans, disease susceptibility is controlled by the classical MHC class II genes that guide CD4(+) T

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