C1919

氯霉素，大包装

BioReagent, suitable for plant cell culture

• 别名: D-(−)-苏-2,2-二氯-N-[β-羟基-α-(羟甲基)-β-(4-硝基苯基)乙基]乙酰胺, D-(−)-苏-2-二氯乙酰胺基-1-(4-硝基苯基)-1,3-丙二醇, D-苏-2,2-二氯-N-β[-羟基-α-(羟甲基)-4-硝基苯乙基]乙酰胺, 氯霉素
• 线性分子式: Cl₂CHCONHCH(CH₂OH)CH(OH)C₆H₄NO₂
• CAS号: 56-75-7
• 分子量: 323.13
• Beilstein: 2225532
• EC號碼: 200-287-4
• MDL號碼: MFCD00078159
• 分類程式碼代碼: 51102831
• PubChem物質ID: 24892448
• NACRES: NA.76

属性

• 產品線: BioReagent
• 品質等級: 200
• 形狀: powder
• 技術: cell culture | plant: suitable
• 雜質: Endotoxin, tested
• mp: 149-153 °C (lit.)
• 抗生素活性譜: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• 應用: agriculture
• 作用方式: protein synthesis | interferes
• 儲存溫度: 2-8°C
• SMILES 字串: OC[C@@H](NC(=O)C(Cl)Cl)[C@H](O)c1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C11H12Cl2N2O5/c12-10(13)11(18)14-8(5-16)9(17)6-1-3-7(4-2-6)15(19)20/h1-4,8-10,16-17H,5H2,(H,14,18)/t8-,9-/m1/s1
• InChI 密鑰: WIIZWVCIJKGZOK-RKDXNWHRSA-N
• 基因資訊: human ... CYP1A2(1544)

说明

一般說明

Chemical structure: phenicole

應用

Chloramphenicol is a synthetic antibiotic, isolated from strains of Streptomyces venezuelae. It is often used for bacterial selection in molecular biology applications at 10-20 μg/mL and as a selection agent for transformed cells containing chloramphenicol reistance genes.

生化／生理作用

作用机制：氯霉素通过与50S核糖体亚基结合并阻止氨酰基tRNA附着于核糖体来阻断肽基转移酶步骤，从而抑制细菌蛋白质的合成。 它还抑制线粒体和叶绿体蛋白的合成以及（p）ppGpp的核糖体形成，从而抑制rRNA的转录。

耐药性机制：使用氯霉素乙酰转移酶会使产物乙酰化并使其失活。

抗菌谱：这是一种针对革兰氏阳性和革兰氏阴性细菌的广谱抗生素，主要用于眼科和兽医目的。

注意

Stock solutions should be stored at 2-8°C and are stable at 37°C for 5 days. Aqueous solutions are neutral and stable over a wide pH range, with 50% hydrolysis occurring after 290 days. Use of a borax buffered solution reduces this number to 14%. Solutions should be protected from light as photochemical decomposition results in a yellowing of the solution. Heating aqueous solutions at 115°C for 30 minutes results in a 10% loss of chloramphenicol.

準備報告

Stock solutions can be prepared directly in the vial at any recommended concentration. A solution at 50 mg/mL in ethanol yields a clear, very faint, yellow solution. Degradation of chloramphenicol in aqueous solution is catalyzed by general acids and bases. This rate of degradation is independent of the ionic strength and pH.

其他說明

Keep container tightly closed in a dry and well-ventilated place, Light sensitive. Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

安全信息

• 象形圖: GHS08,GHS05
• 訊號詞: Danger
• 危險聲明: H318,H351,H361fd
• 防範說明: P202 - P280 - P305 + P351 + P338 - P308 + P313 - P405 - P501
• 危險分類: Carc. 2 - Eye Dam. 1 - Repr. 2
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 個人防護裝備: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges