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生物源
human lung
包裝
pkg of 500,000 cells
形態學
( endothelial)
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
一般說明
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
細胞系來源
Lungs
應用
Primary Human Pulmonary Microvascular Endothelial Cells (HPMEC) are isolated from the lung from a single donor and are provided in a cryopreserved format. Since lung tissue contains blood and lymphatic capillaries, HPMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and von Willebrand factor and negative for smooth muscle alpha-actin.The lung has a vast endothelial surface area, which is essential for the exchange of gases. HPMEC are most appropriate for studying human lung diseases.
品質
Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
警告
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
例行更新培養
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其他說明
Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15
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Recommended Primary Cell Culture Media:Link
免責聲明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Lili Li et al.
Pulmonary circulation, 10(4), 2045894020941352-2045894020941352 (2021-01-16)
While it is well established that the haemodynamic cause of hypoxic pulmonary hypertension is increased pulmonary vascular resistance, the molecular pathogenesis of the increased resistance remains incompletely understood. Macrophage migration inhibitory factor is a pleiotropic cytokine with endogenous tautomerase enzymatic
实验方案
Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.
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