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Merck

C0794

Sigma-Aldrich

壳聚糖酶 来源于链霉菌

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

别名:

壳聚糖N-乙酰氨基葡糖水解酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

buffered aqueous glycerol solution

比活性

≥15 units/mg protein (E1%)

濃度

≥0.5 mg/mL

溶解度

0.1 M acetate buffer, pH 5.5: soluble

儲存溫度

−20°C

相关类别

應用

Sigma的壳聚糖酶已用于几丁质和壳聚糖的偏振光刺激的酶促水解。

生化/生理作用

壳聚糖酶催化壳聚糖中D-氨基葡萄糖(GlcN-GlcN)残基之间的β-1,4-键的内水解。据报道,来自链霉菌属的酶还能水解部分乙酰化壳聚糖中的GlcNAc-GlcN键。

單位定義

一个酶活性单位是指在37 °C、pH 5.5下,每分钟从壳聚糖中释放1.0 μmole还原糖(以D-葡萄糖胺当量计)所需的酶量。

外觀

溶于含50 mM乙酸钠的50%甘油溶液中

準備報告

溶于10 mL的0.1 M乙酸缓冲液,pH值为5.5

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Anna Konieczna-Molenda et al.
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
A new reaction for colorimetric determination of carbohydrates.
M Lever
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
T Fukamizo et al.
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
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