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Key Documents

C-39890

Sigma-Aldrich

Hematopoietic Progenitor Expansion Medium DXF

Cytokine Mix E for 100 ml

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About This Item

分類程式碼代碼:
12352207

形狀

aqueous solution

包裝

pkg of 1 vial

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Cytokine Mix E for HPC-Expansion Medium DXF

應用

Cytokine Mix E is a ready-to-use mixture of growth factors optionally available for the HPC Expansion Medium DXF (C-28021). The animal component-free, defined formula contains recombinant human TPO, SCF, flt-3 ligand and IL-3. The optimized formulation allows vigorous expansion of hematopoietic progenitor cells, while maintaining their primitive phenotype. Differentiation is largely inhibited.

品質

All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

儲存和穩定性

Store the Cytokine Mix at -20°C in the dark immediately after arrival. If stored properly, the it is stable until the expiry date stated on the label. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

Mononuclear cells isolated from umbilical cord blood and adult peripheral blood and maintenance medium optimized for short term cultivation. Protocols for handling, cell passaging, media, and product use.

实验方案

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human CD34+ progenitor cells and xeno-free, defined media for the expansion of hematopoietic progenitor cells. Protocols for handling, cell passaging, media, and product use.

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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