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一般說明
兔醛缩酶存在三种亚型,包括来自肌肉的A型、肝脏B型和大脑相关C型。醛缩酶分子量为158 kDa,以四聚体形成存在。
應用
兔肌肉醛缩酶已用于:
- 在标准兔肌肉来源1-磷酸果糖激酶 (RPFK-1)实验中使用
- 作为青光眼组织来源代谢酶测定的标准品
- 用于人细胞系的果糖2,6-二磷酸分析
醛缩酶可用于将果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。来自兔肌肉中的醛缩酶已被用于DHAP C3的立体特异性去质子化。
生化/生理作用
醛缩酶与Wiskott-Aldrich综合征蛋白(WASP)相互作用,可调节肌动蛋白动态。它可逆转抗坏血酸对肌肉型乳酸脱氢酶(LDH-m4)的抑制作用。
醛缩酶可参与到糖原异生、卡尔文循环和糖酵解。来自兔肌肉中的醛缩酶属于I类醛缩酶,可形成共价席夫碱中间体。醛缩酶的活性位点位于α/β 8 筒形褶皱 的中央。
單位定義
在pH 7.4、25 °C条件下,一个单位可在每分钟内将1.0 μ摩尔的的果糖1,6-二磷酸转化为磷酸二羟丙酮和3-磷酸甘油醛。
外觀
2.5M (NH4)2SO4、0.01M Tris 溶液中的结晶悬浮液,pH 7.5,含 0.001M EDTA
分析報告
由缩二脲测定的蛋白质。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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Instructions for working with enzymes supplied as ammonium sulfate suspensions
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