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Merck

A8311

Sigma-Aldrich

消泡剂 204

aqueous emulsion for bacterial and mammalian systems

别名:

organic antifoam

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About This Item

MDL號碼:
分類程式碼代碼:
12161901
NACRES:
NA.85

品質等級

形狀

emulsion (aqueous)

濃度

100% (organic)

密度

1.01 g/mL at 25 °C

應用

microbiology

儲存溫度

room temp

適合性

(Mammalian (suspension))
bacteria (fermentation)

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一般說明

消泡剂204是一种有机消泡剂,可用于防止或最小化微生物发酵系统和哺乳动物悬浮培养物中的起泡现象。 控制发泡可以最大限度地减少对细胞的损害,从而增加蛋白质/抗体的产生。

應用

适用于防止或充分减少微生物发酵系统和哺乳动物悬浮培养物中的泡沫。
消泡剂204已用于:
  • PUL(PLAP、Ufd3p和Lub1p)结构域的表达和纯化
  • 分批补料发酵
  • 防止Luria Bertani细菌生长培养基中因曝气形成气泡
  • 采用矿物培养基分批发酵

成分

消泡剂204是一种100%活性成分,是一种在多元醇分散体中无硅有机消泡剂的混合物。

其他說明

包含 100% 活性成分,是多种无硅酮有机消泡剂的混合物,在多元醇中分散。可反复灭菌。

準備報告

对于在微生物培养基中的使用,Sigma建议起始浓度为0.005%至0.01%。
各种应用所需的最佳消泡剂用量需经过确定。消泡剂204在低于15℃的温度下可溶于甲醇、乙醇、甲苯、二甲苯、全氯乙烯和冷水中。它不溶于温水和乙二醇。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Yixin Yang et al.
Biomacromolecules, 11(1), 259-268 (2009-12-17)
Biobased omega-carboxy fatty acid monomers 1,18-cis-9-octadecenedioic, 1,22-cis-9-docosenedioic, and 1,18-cis-9,10-epoxy-octadecanedioic acids were synthesized in high conversion yields from oleic, erucic and epoxy stearic acids by whole-cell biotransformations catalyzed by C. tropicalis ATCC20962. Maximum volumetric yields in shake-flasks were 17.3, 14.2, and
Beth Junker
Biotechnology progress, 23(4), 767-784 (2007-06-15)
Key aspects of foaming and its mitigation in fermentation systems are presented. Foam properties and behavior, conditions that affect foaming, and consequences of foaming are discussed, followed by methods to detect and prevent foam, both without and with the use
Medium redesign for stable cultivation and high production of mevalonate by recombinant Methtylobacterium extorquens AM1 with mevalonate synthetic pathway
Cui L Y, et al.
Biochemical Engineering Journal, 119, 67-73 (2017)
Gheorghe M Borja et al.
Microbial cell factories, 18(1), 191-191 (2019-11-07)
Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this
Junpeng Xiao et al.
Methods in molecular biology (Clifton, N.J.), 751, 329-342 (2011-06-16)
Site-specific modification of glycoproteins has wide application in both biochemical and biophysical studies. This method describes the conjugation of synthetic molecules to the N-terminus of a glycoprotein fragment, viz., human immunoglobulin G subclass 1 fragment crystallizable (IgG1 Fc), by native

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