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Merck

A8011

Sigma-Aldrich

消泡剂C乳液

aqueous-silicone emulsion

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About This Item

分類程式碼代碼:
12161900
NACRES:
NA.25

生物源

synthetic

品質等級

形狀

emulsion

包含

emulsifier

技術

cell culture | hybridoma: suitable
microbiological culture: suitable

mp

0  °C (32 °F)

一般說明

消泡剂C乳液是含有30%活性硅的水性乳液。这种基于硅胶的消泡剂乳液含有不同于消泡剂乳液B和Y-30的非离子乳化剂。消泡剂C可以用3-10份冷水预稀释以帮助分散。预稀释后的悬浮液应立即使用。消泡剂C乳液通常在1-10 ppm时有效。消泡剂C乳液良好的流动性使得它可以根据需要泵入发酵罐,同时充分搅拌以分散消泡剂。消泡剂C乳液可用于控制哺乳动物细胞培养生物反应器顶部空间的起泡,最高使用浓度30 ppm。

應用

消泡剂C已用于:
  • 添加到RO-H2O和化学成分确定培养基中,用于测定哺乳动物细胞培养物中的体积传质系数(kLa)
  • 作为补料培养基组分
  • 作为生物控制器中的蒸汽灭菌消泡剂,用于pH-stat法分批补料培养
  • 作为分批补料培养基补充剂,模拟典型的大规模培养条件

特點和優勢

  • 最大程度减少生物学系统的相容性问题
  • 低浓度下的高效消泡剂
  • 高效的多功能有机硅消泡剂

其他說明

一种30%聚二甲基硅氧烷精矿水乳状液。
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象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Bernhard Sissolak et al.
Journal of industrial microbiology & biotechnology, 46(8), 1167-1178 (2019-06-09)
Recombinant monoclonal antibodies are predominantly produced in mammalian cell culture bioprocesses. Post-translational modifications affect the micro-heterogeneity of the product and thereby influence important quality attributes, such as stability, solubility, pharmacodynamics and pharmacokinetics. The analysis of the surface charge distribution of

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