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Key Documents

A7979

Sigma-Aldrich

白蛋白 溶液 来源于牛血清

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

别名:

BSA溶液

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.75

生物源

bovine

品質等級

描述

endotoxin tested

無菌

sterile-filtered

產品線

BioXtra

化驗

33-37% total protein basis (UV)

形狀

liquid

來源

USA origin

濃度

35% in DPBS

技術

blocking: suitable
cell culture | mammalian: suitable

雜質

≤1000 EU/mL Endotoxin level

pH值

6.5-7.5

異物活動

Mycoplasma Test by Barile On raw material, none detected

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

BSA是由约583个氨基酸残基组成,不含碳水化合物的单一多肽链。在pH 5-7时,它含有17个链内二硫键和1个巯基。

應用

牛血清白蛋白被广泛用作细胞培养基,特别是无血清培养基的添加剂。 它可带来一系列的好处,包括防止氧化损伤和稳定其他培养基成分,如脂肪酸和吡哆醛。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Neil T Burford et al.
Assay and drug development technologies, 15(7), 320-329 (2017-11-10)
Homogeneous functional assays that utilize competition binding technology are widely used for determining pharmacological properties such as intrinsic activity and potency. One example is time-resolved fluorescence resonance energy transfer (TR-FRET) 3',5'-cyclic adenosine monophosphate (cAMP) assays, where labeled cAMP (tracer) and
Gustavo A Gomez et al.
Developmental biology, 449(2), 99-106 (2019-03-04)
The developmental biology of neural crest cells in humans remains unexplored due to technical and ethical challenges. The use of pluripotent stem cells to model human neural crest development has gained momentum. We recently introduced a rapid chemically defined approach
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
John P Ray et al.
Nature communications, 11(1), 1237-1237 (2020-03-08)
Genome-wide association studies have associated thousands of genetic variants with complex traits and diseases, but pinpointing the causal variant(s) among those in tight linkage disequilibrium with each associated variant remains a major challenge. Here, we use seven experimental assays to
Bryne Ulmschneider et al.
The Journal of cell biology, 215(3), 345-355 (2016-11-09)
Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle

商品

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。 这些深度知识可能也适用于离体和组织工程学应用。

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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