A3156

牛血清白蛋白 来源于牛血清

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

• 别名: BSA, 牛白蛋白, 牛血清白蛋白
• CAS号: 9048-46-8
• EC號碼: 232-936-2
• MDL號碼: MFCD00130384
• 分類程式碼代碼: 12352202
• NACRES: NA.75

属性

• 生物源: bovine
• 品質等級: 200
• 無菌: γ-irradiated
• 產品線: BioXtra
• 化驗: ≥99% (agarose gel electrophoresis)
• 形狀: lyophilized powder
• 分子量: ~66 kDa
• 純化經由: cold ethanol and heatshock fractionation
• 來源: USA origin
• 技術: cell culture | mammalian: suitable
• 雜質: ≤10% water (Karl Fischer)
• pH值: 4.8-7.5
• 溶解度: water: soluble (5g/50ml)
• UniProt登錄號: P02769
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: bovine ... ALB(280717)

说明

一般說明

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans.

應用

Bovine serum albumin has been used:

• to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration
• to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay
• in alginate solution as a protein model for drug release kinetics investigation

生化／生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation.

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

準備報告

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

采用热休克和乙醇分级分离相结合的方法进行纯化

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)