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生物源
bacterial (LYSOBACTER SP. BACTERIA M497-1)
品質等級
形狀
lyophilized powder
比活性
≥1,000 units/mg solid
分子量
27 kDa
技術
cell based assay: suitable
適合性
suitable for cell lysis
應用
diagnostic assay manufacturing
運輸包裝
dry ice
儲存溫度
−20°C
應用
消化肽酶可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。Sigma 的消化肽酶可用于皮肤和软组织细菌感染的分离和分子鉴定。
生化／生理作用
消化肽酶是赖氨酰内肽酶，其分子量约 27kDa。它在 pH 8.5-9之间效果最佳。可以使用大约 500-1,500 μn/ml 的无色肽酶裂解细胞，细胞密度 OD600 =0.6，37℃，超过 2 小时。
消化肽酶是赖氨酰内肽酶，分子量约 27kDa。它可用于裂解对溶菌酶具有抗性的革兰氏阳性细菌。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。
活性最佳的 pH 值：pH 8.5-9
可以使用大约 500-1,500ng/ml 的消化肽酶裂解细胞，细胞密度 OD600=0.6，37℃，超过 2 小时 。
單位定義
在 pH8.0，37℃下，使用溶壁微球菌（Micropoccus lysodeikticus）悬浮液作为底物，一个单位的酶每分钟每毫升产生 0.001 的 A600 变化（1cm 光程）。
外觀
粗粉
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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实验方案
Enzymatic Assay of Achromopeptidase
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