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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~36 kDa
物種活性
human, mouse, rat
技術
immunoprecipitation (IP): 5-10 μL using rat NRK cell lysate
western blot: 2-4 μg/mL using whole extracts of human HeLa and mouse 3T3 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ATG3(64422)
mouse ... Atg3(67841)
一般說明
ATG3 is a member of the autophagy-related protein family and is involved in the formation of the autophagosome.
Autophagy-related protein 3 (ATG3) is a member of the autophagy-related protein family. It is a mammalian homolog of yeast Apg3p/ Atg3 is ubiquitously expressed in human tissues.
免疫原
synthetic peptide corresponding to amino acids 17-32 of human Atg3, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
應用
Anti-ATG3 antibody produced in rabbit has been used in western blotting and immunoprecipitation.
生化／生理作用
Autophagy-related protein 3 (ATG3) is involved in the formation of the autophagosome. the mammalian homolog of yeast Apg3p/Aut1p, is an E2-like enzyme that catalyzes the conjugation reaction between Atg8 and phosphatidylethanolamine (PE). Golgi-associated ATPase enhancer of 16 kDa (GATE-16), GABAA receptor-associated protein (GABARAP), and microtubule-associated protein light chain 3 (MAP-LC3) are substrates for Atg3. MAP-LC3 is the preferred substrate. Atg3 interacts with Atg7 to form an E1-E2 complex, and with Atg12, which is a substrate for Atg7 but not for Atg3. Moreover, overexpression of Atg3 facilitates the formation of the Atg12-Atg5 conjugate.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Human ATG4 autophagy proteases counteract attachment of ubiquitin-like LC3/GABARAP proteins to other cellular proteins
Agrotis A, et al.
The Journal of Biological Chemistry, 294(34), 12610-12621 (2019)
ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function
Murrow L, et al.
Nature Cell Biology, 17(3), 300-300 (2015)
Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12
Metlagel Z, et al.
Proceedings of the National Academy of Sciences of the USA, 110(47), 18844-18849 (2013)
The transcription and translation landscapes during human cytomegalovirus infection reveal novel host-pathogen interactions
Tirosh O, et al.
PLoS Pathogens, 11(11), e1005288-e1005288 (2015)
Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p
Tanida I, et al.
Test, 277(16), 13739-13744 (2002)
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