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71028

Sigma-Aldrich

单溴（三甲基铵）二溴化溴

suitable for fluorescence, ≥90.0% (HPLC)

别名:

5-(溴甲基) -N,N,N,2,6-五甲基-1,7-二氧代-1H,7H-吡唑并[1,2-A]吡唑-3-甲基溴化铵, Brombimane q, qBBr, 硫醇MQ, （三甲基铵）单溴二烯

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About This Item

经验公式（希尔记法）:
C13H19Br2N3O2
CAS号:
71418-45-6
分子量:
409.12
Beilstein:
4630658
MDL號碼:
MFCD00036953
分類程式碼代碼:
12171500
PubChem物質ID:
57652269
NACRES:
NA.32

化驗

≥90.0% (HPLC)

形狀

powder

溶解度

DMSO: 5 mg/mL

螢光

λex 380 nm; λem 475 nm in 0.1 M phosphate pH 7.5

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

[Br-].CC1=C(CBr)N2N(C1=O)C(=O)C(C)=C2C[N+](C)(C)C

InChI

1S/C13H19BrN3O2.BrH/c1-8-10(6-14)15-11(7-17(3,4)5)9(2)13(19)16(15)12(8)18;/h6-7H2,1-5H3;1H/q+1;/p-1

InChI 密鑰

FCRPMMVPBVOKQS-UHFFFAOYSA-M

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生化／生理作用

在与低分子量硫醇发生共轭反应之前，单溴二胺基本上是不发荧光的。与普通的单溴二胺不同，它带有正电荷，因此可以通过电泳或阳离子交换色谱法分离缀合物

包裝

无底玻璃瓶。内含物在插入的融合锥体内。

其他說明

用于标记生物材料中硫醇的试剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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S X Yuan et al.
FEBS letters, 234(1), 218-222 (1988-07-04)
The single cysteinyl residue 26 of spinach calmodulin was labelled with the thiol-specific bimane fluorescence probe. Following application of stoichiometric quantities of Ca2+ or aluminum ions to the protein, temperature-dependent fluorescence changes (anisotropy, lifetime) could be monitored via the label.
S S Ristow et al.
Immunological investigations, 14(5), 401-414 (1985-10-01)
Cell surface thiols are required for a line of cloned murine natural killer lymphocytes to bind to and lyse tumor target cells. These lymphocytes neither bound to nor killed YAC-1 or G1Tc cells when the effector lymphocyte cell surface thiols
M Balázs et al.
Biochemical and biophysical research communications, 140(3), 999-1006 (1986-11-14)
The accessibility of cell surface sulfhydryl groups in human peripheral lymphocytes was investigated with 5,5'-dithiobis-(2-nitrobenzoic acid) in the presence and absence of ionophore antibiotics and the monoclonal antibody, OKT-3. Only a few accessible protein thiols have been found on the
Claresta Joe-Wong et al.
Environmental science & technology, 46(18), 9854-9861 (2012-08-25)
Organic thiols are highly reactive ligands and play an important role in the speciation of several metals and organic pollutants in the environment. Although small thiols can be isolated and their concentrations can be estimated using chromatographic and derivatization techniques
J Lahav et al.
FEBS letters, 475(2), 89-92 (2000-06-20)
Cell adhesion is mediated by the integrin adhesion receptors. Receptor-ligand interaction involves conformational changes in the receptor, but the underlying mechanism remains unclear. Our earlier work implied a role for sulfhydryls in integrin response to ligand binding in the intact
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