66939

Phalloidin–Atto 425

suitable for fluorescence, ≥90% (HPLC)

• 别名: Atto 425–Phalloidin
• 分類程式碼代碼: 12352116
• NACRES: NA.32

属性

• 化驗: ≥90% (HPLC)
• 製造商／商標名: ATTO-TEC GmbH
• &lambda ；: in methanol
• 紫外吸收: λ: 429-435 nm A_max
• 適合性: suitable for fluorescence
• 儲存溫度: −20°C

说明

一般說明

Atto 425 Phalloidin is a novel fluorescent label with a coumarin structure. The dye is intended for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, large Stokes-shift, good photostability, and low molecular weight.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.

應用

Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 425 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)