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Key Documents

63301-011

Sigma-Aldrich

PCRboost®

1 mL bottle, for improved PCR performance

别名:

PCRboost®

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.52

形狀

liquid

用途

28 reactions

儲存溫度

room temp

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一般說明

PCRboost® is a unique reagent that enhances end-point and reverse transcription-PCR performance by improving sensitivity and specificity during amplification of genomic DNA or RNA templates. To use, simply replace the water in your PCR with PCRboost®. That′s all. It is designed to enhance the amplification of DNA without the need for time consuming optimizations. PCRboost® successfully amplifies thermally degraded DNA resulting in a visible amplicon that can be used for downstream applications such as cloning and sequencing.

Don′t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available through Sigma′s partner, Biomatrica. PCRstable® enables the preservation of PCR-based assay components in any format (liquid or dry).

特點和優勢

Boost PCR results for:

  • Degraded FFPE samples and bisulphite-treated samples (methylation studies)
  • Multiplex PCR
  • Low-copy transcripts
  • Samples containing inhibitory factors (i.e. humic acid, heme, and indigo dye)

No changes to your PCR protocol. Simply replace the water in 
your reaction.

PCR products are compatible with all downstream applications.

包裝

1 bottle

外觀

1.0 mL PCRboost® in a 1.5 mL screw-top conical tube

法律資訊

PCRboost is a registered trademark of Biomatrica
PCRstable is a registered trademark of Biomatrica

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions.

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