350R-1

FoxP1 (SP133) Rabbit Monoclonal Antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100; 500
• 共軛: unconjugated
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: SP133, monoclonal
• 描述: For In Vitro Diagnostic Use in Select Regions (See Chart)
• 形狀: buffered aqueous solution
• 物種活性: human
• 包裝: vial of 0.1 mL concentrate (350R-14); vial of 0.5 mL concentrate (350R-15); bottle of 1.0 mL predilute (350R-17); vial of 1.0 mL concentrate (350R-16); bottle of 7.0 mL predilute (350R-18)
• 製造商／商標名: Cell Marque^®
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• 同型: IgG
• 控制: DLBCL, lymph node, tonsil
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 視覺化: nuclear
• 基因資訊: human ... FOXP1(27086)

说明

一般說明

Diffuse large B-cell lymphoma (DLBCL) represents different clinicopathologic entities which are difficult to separate using standard techniques. From the clinical standpoint, the introduction of immunochemotherapy in the treatment of DLBCL has dramatically improved the outcome of these patients compared with chemotherapy alone. Gene expression profiling (GEP) studies have shown that DLBCL can be reproducibly divided into the important subtypes of germinal center B-cell–like (GCB), activated B-cell–like (ABC), and unclassified DLBCL. It is beneficial to translate the GEP classification into protein expression by tumor cells through immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded tissues. A panel of antibodies: CD10, BCL6, MUM1/IRF4, GCET1, FoxP1, LMO2, and BCL2 has been used to determine GCB or ABC and each has different percentage thresholds for positive staining. Choi et al. demonstrated that the cases positive for GCET1 (≥ 80% of tumor cells) and MUM1/IRF4 (≥ 80%) and/or FoxP1 (≥ 80%) or negative for CD10 and BCL6 (≤ 30%) were assigned to the group. The cases positive for CD10 (≥ 30%), GCET1 (≥ 80%) without MUM1 expression, or positive for BCL6 without FoxP1 expression were classified as GCB. This study indicated the importance of FoxP1 in the subclassification of DLBCL. Choi et al then modified their approach to DLBCL subclassification by focusing on FoxP1. The tumors that are positive for both FoxP1 and GCET1 are assigned to GCB subgroup, but, if FoxP1 is positive and GCET1 is negative, the tumors belong to the ABC phenotype. If a case is FoxP1 negative but MUM-1/IRF4 positive, it still belongs to the ABC phenotype as long as CD10 is not expressed. This modified method emphasized the role of FoxP1, MUM1/IRF4, and GCET1 in the subclassification of DLBCL. The Choi′s algorithm had a very high concordance with the GEP results (87%). Therefore, FoxP1 is useful in subclassification of DLBCL and a high cutoff (≥80%) for FoxP1 is needed to achieve high specificity for the ABC subtype.

品質

IVD

IVD

IVD

RUO

聯結

FoxP1 Positive Control Slides, Product No. 350S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

外觀

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

準備報告

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

其他說明

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法律資訊

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany