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Merck

07679

Sigma-Aldrich

Abberior® STAR 635P, NHS ester

suitable for fluorescence

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About This Item

分類程式碼代碼:
12352108
NACRES:
NA.32

分子量

Mw 1030 g/mol

溶解度

DMF: 0.25 mg/mL, clear

螢光

λex 634 nm; λem 654 nm±10 nm in PBS, pH 7.4

適合性

suitable for fluorescence

儲存溫度

−20°C

一般說明

Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.
Abberior STAR 635P can substitute Atto 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.
Best results are obtained with freshly prepared samples.

Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.

Key Features
  • First phosphorylated dye
  • Unmatched, background free STED imaging contrast
  • Verified in the Leica STED system

Absorption Maximum, λmax: 638 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 120′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 - 780 nm
Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

法律資訊

Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

相關產品

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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