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共軛
Atto 647N conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
包含
50% glycerol as stabilizer
物種活性
mouse
濃度
≥0.8 mg/mL IgG
技術
immunofluorescence: 5 μg/mL
螢光
λex 647 nm; λem 665 nm in PBS
適合性
in accordance for fluorescence
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。Atto 647N山羊抗小鼠IgG与小鼠IgG结合。
免疫原
小鼠 IgG
應用
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性,构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越,光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗(浓度为 5μg/ml),用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1 篇论文)
蛋白质免疫印迹分析(1 篇论文)
外觀
Atto 647 山羊抗小鼠 IgG(全分子)以 1 mg/ml 溶液的形式提供,溶剂为 0.1M 磷酸钠,0.1M NaCl,pH 7.5,含有 5 mM 叠氮化钠作为防腐剂。
分析報告
未缀合染料的总荧光 ≤5%
法律資訊
本产品仅供研究使用。如果要商业化,请联系知识产权持有者(ATTO-TEC GmbH,德国)获取许可。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves
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商品
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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