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產品線
BioReagent
化驗
≥90% (HPCE)
製造商／商標名
ATTO-TEC GmbH
螢光
λex 533 nm; λem 560 nm in 0.1 M phosphate pH 7.0
適合性
suitable for fluorescence
儲存溫度
−20°C
一般說明
Atto 532是一种新的标签，具有高分子吸收（115,000）和量子产率（0.90），并且在激发和发射最大值之间具有足够的斯托克斯′位移。它针对倍频Nd:YAG激光器的激发进行了优化，并具有高光稳定性。
應用
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性，构型不会产生任何顺反异构变化。因此这种标记偶联后的强度表现卓越，光谱转变却又极低。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
Atto 532已与二抗偶联，用于STED（刺激发射耗竭）显微镜和免疫荧光研究。
法律資訊
本产品仅供研究使用。如果打算商业化，请联系知识产权持有者（德国ATTO-TEC GmbH公司）申请许可。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Uffe V Schneider et al.
BMC biotechnology, 10, 4-4 (2010-01-28)
Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV
3D reconstruction of high-resolution STED microscope images.
Punge A et al.
Microscopy Research and Technique, 71, 644-644 (2008)
Evidence for major structural changes in subunit C of the vacuolar ATPase due to nucleotide binding.
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit
Daniel Aquino et al.
Nature methods, 8(4), 353-359 (2011-03-15)
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in
SERRS-based detection of dye-labeled DNA using positively-charged Ag nanoparticles.
Gill, R. and Lucassen, G. W.
Analytical Methods : Advancing Methods and Applications, 2, 445-447 (2010)
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