推荐产品
無菌
non-sterile
品質等級
產品線
BioChemika
形狀
powder
儲存期限
limited shelf life, expiry date on the label
製造商／商標名
NutriSelect® Plus
儲存條件
protect from light at room temperature
技術
microbe id | susceptibility testing: suitable
microbiological culture: suitable
最終pH值
6.6±0.2 (25 °C)
應用
clinical testing
food and beverages
life science and biopharma
veterinary
microbiology
適合性
mycobacteria
一般說明
Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria.
應用
There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein’s formulation) and agar-based (Middlebrook formulation)
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
The latter has the following advantages over the egg-based media
The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
There is less inactivation of added drugs when egg ingredients are absent
成分
成分 (g/L)
酶水解酪蛋白，1.00
硫酸铵，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
硫酸镁，0.05
L-谷氨酸，0.50
柠檬酸铁铵，0.04
吡哆素，0.001
生物素，0.0005
孔雀绿，0.001
琼脂，15.00
酶水解酪蛋白，1.00
硫酸铵，0.50
磷酸二氢钾，1.50
磷酸二钠，1.50
柠檬酸钠，0.40
硫酸镁，0.05
L-谷氨酸，0.50
柠檬酸铁铵，0.04
吡哆素，0.001
生物素，0.0005
孔雀绿，0.001
琼脂，15.00
準備報告
Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.
腳註
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律資訊
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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