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Merck

68022

Millipore

Y. enterocolitica Millichrome plus Supplement

suitable for microbiology

别名:

Y. enterocolitica chromogenic supplement

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About This Item

分類程式碼代碼:
41171614
NACRES:
NA.74

無菌

sterile; 0.45 μm filtered

品質等級

形狀

dry powder

儲存期限

limited shelf life, expiry date on the label

技術

microbiological culture: suitable

溶解度

sterile water: 100 mg/mL

應用

food and beverages
microbiology

運輸包裝

ambient

儲存溫度

2-8°C

適合性

selective for bacteria: Yersinia enterocolitica

一般說明

Y. enterocolitica Millichrome plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vials sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome plus Agar base cooled at 45-50 °C +/- 2 °C.

應用

Y. enterocolitica Millichrome plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.

成分

Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g

準備報告

  • Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
  • Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
  • Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
  • Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome plus Agar base cooled at 45-50 °C +/- 2 °C.
  • Swirl gently to homogenize.
  • Pour into sterile Petri dishes.

分析報告

The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature

法律資訊

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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