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無菌
sterile; 0.45 μm filtered
品質等級
形狀
dry powder
儲存期限
limited shelf life, expiry date on the label
技術
microbiological culture: suitable
溶解度
sterile water: 100 mg/mL
應用
food and beverages
microbiology
運輸包裝
ambient
儲存溫度
2-8°C
適合性
selective for bacteria: Yersinia enterocolitica
一般說明
Y. enterocolitica Millichrome™ plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vials sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
應用
Y. enterocolitica Millichrome™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.
成分
Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
準備報告
- Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
- Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
- Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
- Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
- Swirl gently to homogenize.
- Pour into sterile Petri dishes.
分析報告
The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature
法律資訊
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
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