07689

O'Meara 试剂

suitable for microbiology

• 别名: 使用 O'Meara 试剂的 Voges-Proskauer 试验
• 分類程式碼代碼: 41171621
• NACRES: NA.85

属性

• 產品線: BioChemika
• 品質等級: 100
• 儲存期限: limited shelf life, expiry date on the label
• 成份: creatine, 0.3 g ; dist. water, 100 mL ; potassium hydroxide, 40 g
• 包裝: pkg of 100 mL
• 儲存條件: (Tightly closed. Dry.)
• 技術: microbe id | metabolite detection: suitable
• 應用: environmental; food and beverages; microbiology
• 適合性: Enterobacter spp.; Klebsiella spp.; Neisseria spp.; Pseudomonas spp.

说明

一般說明

O′Meara′s Reagent is used in the Voges-Proskauer (VP) test for the detection of acetoin production by bacterial strains. VP test is helpful in identifying members of the family Enterobacteriaceae based on their ability to produce acetylmethylcarbinol (AMC = acetonin) from glucose. Acetoin is an intermediate which is oxidized by oxygen under alkaline conditions (KOH) to 2,3-Butanediol. With creatinine (break down product from creatine) and the enhancement of α-naphtol a red color is formed (Meisenheimer complex)

應用

The O′Meara reagent is a component of the Voges-Proskauer (VP) test, which is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce acetylmethylcarbinol (AMC) from glucose. The VP test is often used as a tool to identify and authenticate bacterial strains in the food, beverage, and pharmaceutical industries.

分析報告

To perform the VP test, a bacterial sample is inoculated into a nutrient broth and incubated. The O′Meara reagent, which contains a mixture of creatinine and potassium hydroxide (KOH), is then added to the culture. Acetoin produced in the medium by bacterial culture is oxidized under alkaline conditions in the presence of air to form diacetyl which reacts with creatine to give a pinkish-red compound.

安全信息

• 象形圖: GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H290,H302,H314
• 防範說明: P234 - P270 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
• 儲存類別代碼: 8B - Non-combustible corrosive hazardous materials
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter