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Merck

11922416001

Roche

抗组氨酸6

from mouse IgG1

别名:

金属螯合亲合性层析法

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About This Item

分類程式碼代碼:
12352203

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

monoclonal

形狀

lyophilized

包裝

pkg of 100 μg

製造商/商標名

Roche

同型

IgG1

儲存溫度

2-8°C

一般說明

Anti-His 6 是针对 His 6 标签蛋白的单克隆抗体。抗 His 6 抗体特异性识别天然蛋白和重组蛋白的 6 个连续组氨酸残基的抗原表位。抗体与天然和变性组氨酸标记的融合蛋白反应,与蛋白序列中组氨酸标记的位置无关。

特異性

抗 His 6 抗体特异性识别天然和重组蛋白中 6 个连续组氨酸残基 (His 6 ) 的抗原表位。它与天然的和变性的组氨酸标记的融合蛋白反应,与蛋白序列中组氨酸标记的位置无关。此外,抗 His 6 抗体优先识别具有高灵敏度的 C 末端 His 6 表位。无论使用何种表达系统,Anti-His 6 均可特异性且灵敏地检测组氨酸标记蛋白。
该抗体识别天然和重组蛋白中 6 个连续组氨酸残基 (His6) 的抗原表位。它与天然的和变性的组氨酸标记的融合蛋白反应,与表位序列位置无关。

應用

Anti-His 6 用于检测 His 6 标记的蛋白:
  • 免疫印迹(例如:斑点印迹和免疫印迹)
  • 免疫沉淀
  • 免疫分析 (ELISA)
  • 免疫细胞化学
  • 免疫荧光

準備報告

稳定剂: BSA
工作浓度: 结合物的工作浓度取决于应用和底物。
以下浓度应作为指导:
  • 免疫分析:检测为 0.1μg/ml;涂覆为 1-5μg/ml
  • 免疫沉淀:0.5~5μg/ml
  • Western 印迹法:0.2~0.5μg/ml

重構

加入 1 mL 双蒸水至终浓度 100 μg/ml。

其他說明

仅用于生命科学研究。不可用于诊断。

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象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Aquatic Chronic 3 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

does not flash

閃點(°C)

does not flash


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