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Merck

11699075001

Roche

Nylon Membranes for Colony and Plaque Hybridization

别名:

membrane

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About This Item

分類程式碼代碼:
41105339

包裝

pkg of 50 discs (82 mm diameter)

品質等級

製造商/商標名

Roche

盤直徑

82 mm

儲存溫度

20-25°C

一般說明

Nylon membrane is a circular, uncharged, microporous, amphoteric, ultra-pure nylon 66 membrane on a polyester support. It has a durable structure with consistent transfer rate.
Surface Properties: Pore size of 1.2 μm. Surface contains 50% amino and 50% carboxyl groups.

應用

  • The membrane discs are specifically suited for:Nonradioactive screening of phage or cosmid libraries with digoxigenin-labeled probes followed by detection with highly sensitive chemiluminescent or chromogenic substrates
  • Hybridization with biotinylated or radioactive probes
  • It has been used in the colony immunoblotting technique.

其他說明

仅用于生命科学研究。不可用于诊断。

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K P Karey et al.
Analytical biochemistry, 178(2), 255-259 (1989-05-01)
Western blotting of low molecular weight (Mr) acidic and basic isoelectric point (pI) proteins was studied to optimize detection sensitivities. Radioiodinated epidermal growth factor (EGF, Mr 6045, pI 4.4), transforming growth factor type alpha (TGF alpha, Mr 5623, pI 6.8)

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